unc13b shrna lentiviral vector Search Results


unc13b shrna lentiviral vector  (Addgene inc)
96
Addgene inc unc13b shrna lentiviral vector
Elevated expression of <t>UNC13B</t> in Wilms’ tumor cell lines. (A) Western blot analysis of UNC13B expression in WT-CLS1, 17.94, G401, SK-NEP-1 and HK-2 cells. Each lane was loaded with 20 µg protein and GAPDH was used as a reference. (B) Analysis of UNC13B transcription levels in different cell lines; n=5. (C) Changes in UNC13B transcription levels 48 h post-shRNA-mediated UNC13B knockdown in 17.94 cells compared with the scramble control; n=5. (D) UNC13B expression changes 48 h post-shRNA-mediated knockdown in 17.94 cells and (E) statistical analysis of the expression level changes. Each experiment was repeated 3 times, with GAPDH used as a reference. (F) Assessment of cell proliferation post-knockdown using a Cell Counting Kit-8 assay, measuring OD450 values at different time points; n=3. Cells were also transfected with non-target scrambled shRNA as a control. ***P<0.001; ****P<0.0001. UNC13B, unc-13 homolog B; sh, short hairpin; OD, optical density.
Unc13b Shrna Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc13b+shrna+lentiviral+vector/shRNA+(Plasmid+%2355783)/pmc11292464-51-12-22
Average 96 stars, based on 1 article reviews
unc13b shrna lentiviral vector - by Bioz Stars, 2026-09
96/100 stars
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lentiviral vector lenticrispr v2  (Addgene inc)
96
Addgene inc lentiviral vector lenticrispr v2
Effect of UNC13B gene ablation on the cell cycle in ARD cells. Five days after transduction with shRNA <t>lentiviral</t> vectors, the shUNC13B group showed a reduced fraction of ARD cells in G1 ( p < 0.0001) and an increased fraction in S phase ( p < 0.0001) compared with the Scramble control, with no significant change in G2/M (ns, not significant). Histograms show DNA-content modeling: red areas denote fitted G1 and G2/M peaks, the blue hatched area denotes S phase, and the black line is the measured distribution; ▲ indicates software-generated peak positions (reference only, not used for quantification). **** indicate p < 0.0001.
Lentiviral Vector Lenticrispr V2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc13b+shrna+lentiviral+vector/lentiCRISPR+v2+(Plasmid+%2352961)/pmc12467950-38-26-30
Average 96 stars, based on 1 article reviews
lentiviral vector lenticrispr v2 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


Elevated expression of UNC13B in Wilms’ tumor cell lines. (A) Western blot analysis of UNC13B expression in WT-CLS1, 17.94, G401, SK-NEP-1 and HK-2 cells. Each lane was loaded with 20 µg protein and GAPDH was used as a reference. (B) Analysis of UNC13B transcription levels in different cell lines; n=5. (C) Changes in UNC13B transcription levels 48 h post-shRNA-mediated UNC13B knockdown in 17.94 cells compared with the scramble control; n=5. (D) UNC13B expression changes 48 h post-shRNA-mediated knockdown in 17.94 cells and (E) statistical analysis of the expression level changes. Each experiment was repeated 3 times, with GAPDH used as a reference. (F) Assessment of cell proliferation post-knockdown using a Cell Counting Kit-8 assay, measuring OD450 values at different time points; n=3. Cells were also transfected with non-target scrambled shRNA as a control. ***P<0.001; ****P<0.0001. UNC13B, unc-13 homolog B; sh, short hairpin; OD, optical density.

Journal: Oncology Letters

Article Title: UNC13B regulates the sensitivity of Wilms' tumor cells to doxorubicin by modulating lysosomes

doi: 10.3892/ol.2024.14579

Figure Lengend Snippet: Elevated expression of UNC13B in Wilms’ tumor cell lines. (A) Western blot analysis of UNC13B expression in WT-CLS1, 17.94, G401, SK-NEP-1 and HK-2 cells. Each lane was loaded with 20 µg protein and GAPDH was used as a reference. (B) Analysis of UNC13B transcription levels in different cell lines; n=5. (C) Changes in UNC13B transcription levels 48 h post-shRNA-mediated UNC13B knockdown in 17.94 cells compared with the scramble control; n=5. (D) UNC13B expression changes 48 h post-shRNA-mediated knockdown in 17.94 cells and (E) statistical analysis of the expression level changes. Each experiment was repeated 3 times, with GAPDH used as a reference. (F) Assessment of cell proliferation post-knockdown using a Cell Counting Kit-8 assay, measuring OD450 values at different time points; n=3. Cells were also transfected with non-target scrambled shRNA as a control. ***P<0.001; ****P<0.0001. UNC13B, unc-13 homolog B; sh, short hairpin; OD, optical density.

Article Snippet: The negative controls are scramble sequences synthesized by igebio biotech Co., Ltd. UNC13B shRNA lentiviral vector using lentiCRISPR v2. (cat. no. 52961; Addgene, Inc.) was constructed.

Techniques: Expressing, Wilms Tumor Assay, Western Blot, shRNA, Knockdown, Control, Cell Counting, Transfection

UNC13B influences Wilms’ tumor sensitivity to chemotherapy drugs independent of the cell cycle. Evaluation of cell proliferation post-shRNA-mediated UNC13B knockdown after treatment with varying concentrations of (A) vincristine, (B) actinomycin-D and (C) doxorubicin for 48 h in 17.94 cells, and (D) vincristine, (E) actinomycin-D and (F) doxorubicin in the G401 cell line, assessed using Cell Counting Kit-8 assays. Changes in drug sensitivity were analyzed, with dashed lines representing fitted curves for half-maximal inhibitory concentration calculated using GraphPad software, and the cell number ratio indicating the relative number of viable cells compared between initial cell number and different time points. (G) Cell cycle analysis of 1 µM doxorubicin treatment on control and shUNC13B knockdown cells, detected after 48 h post-drug treatment. (H) Quantification of the G1, S and G2 phases of the scramble and shUNC13B groups. (I) Typical pseudocolor scatter plots of the apoptosis analysis of UNC13B-knockdown 17.94 cells after 48 h treatment with 0.5 and 2 µM doxorubicin, and (J) statistical results. **P<0.01; ***P<0.001; ****P<0.0001. UNC13B, unc-13 homolog B; sh, short hairpin.

Journal: Oncology Letters

Article Title: UNC13B regulates the sensitivity of Wilms' tumor cells to doxorubicin by modulating lysosomes

doi: 10.3892/ol.2024.14579

Figure Lengend Snippet: UNC13B influences Wilms’ tumor sensitivity to chemotherapy drugs independent of the cell cycle. Evaluation of cell proliferation post-shRNA-mediated UNC13B knockdown after treatment with varying concentrations of (A) vincristine, (B) actinomycin-D and (C) doxorubicin for 48 h in 17.94 cells, and (D) vincristine, (E) actinomycin-D and (F) doxorubicin in the G401 cell line, assessed using Cell Counting Kit-8 assays. Changes in drug sensitivity were analyzed, with dashed lines representing fitted curves for half-maximal inhibitory concentration calculated using GraphPad software, and the cell number ratio indicating the relative number of viable cells compared between initial cell number and different time points. (G) Cell cycle analysis of 1 µM doxorubicin treatment on control and shUNC13B knockdown cells, detected after 48 h post-drug treatment. (H) Quantification of the G1, S and G2 phases of the scramble and shUNC13B groups. (I) Typical pseudocolor scatter plots of the apoptosis analysis of UNC13B-knockdown 17.94 cells after 48 h treatment with 0.5 and 2 µM doxorubicin, and (J) statistical results. **P<0.01; ***P<0.001; ****P<0.0001. UNC13B, unc-13 homolog B; sh, short hairpin.

Article Snippet: The negative controls are scramble sequences synthesized by igebio biotech Co., Ltd. UNC13B shRNA lentiviral vector using lentiCRISPR v2. (cat. no. 52961; Addgene, Inc.) was constructed.

Techniques: Wilms Tumor Assay, shRNA, Knockdown, Cell Counting, Concentration Assay, Software, Cell Cycle Assay, Control

UNC13B localizes within vesicles and participates in regulating lysosome formation. (A) Indirect immunofluorescence detecting endogenous UNC13B expression in 17.94 cells and revealing UNC13B localization within cellular vesicles. Green fluorescence represents UNC13B and blue fluorescence represents the cell nucleus. (B) Staining of 17.94 cells with Lyso-Tracker. Cells were cultured in confocal culture dishes. The Mean Gray Value of red fluorescence was calculated in 7 different random fields using ImageJ software. Objective, 20X. (C) Mean Gray Value, calculated by measuring the grayscale values in indicated fluorescence channel regions of interest. ****P<0.0001. UNC13B, unc-13 homolog B; sh, short hairpin.

Journal: Oncology Letters

Article Title: UNC13B regulates the sensitivity of Wilms' tumor cells to doxorubicin by modulating lysosomes

doi: 10.3892/ol.2024.14579

Figure Lengend Snippet: UNC13B localizes within vesicles and participates in regulating lysosome formation. (A) Indirect immunofluorescence detecting endogenous UNC13B expression in 17.94 cells and revealing UNC13B localization within cellular vesicles. Green fluorescence represents UNC13B and blue fluorescence represents the cell nucleus. (B) Staining of 17.94 cells with Lyso-Tracker. Cells were cultured in confocal culture dishes. The Mean Gray Value of red fluorescence was calculated in 7 different random fields using ImageJ software. Objective, 20X. (C) Mean Gray Value, calculated by measuring the grayscale values in indicated fluorescence channel regions of interest. ****P<0.0001. UNC13B, unc-13 homolog B; sh, short hairpin.

Article Snippet: The negative controls are scramble sequences synthesized by igebio biotech Co., Ltd. UNC13B shRNA lentiviral vector using lentiCRISPR v2. (cat. no. 52961; Addgene, Inc.) was constructed.

Techniques: Immunofluorescence, Expressing, Fluorescence, Staining, Cell Culture, Software

UNC13B modulates cell drug sensitivity by affecting lysosome formation. (A) 17.94 cell line was transiently transfected with the UNC13B-pCDNA3.1 overexpression vector using Lipofectamine 3,000. Reverse transcription-quantitative PCR validation was performed 24 h post-transfection. NC was the transfection with an empty pcDNA3.1 vector; n=3. (B) UNC13B and LAMP1 expression levels in 17.94 NC cells, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells, using GAPDH as a reference, and (C) the associated semi-quantitative results. (D) Analysis of Mean Gray Value of Lyso-Tracker in 6 random fields of 17.94 NC, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells, and (E) representative images of 17.94 NC cells, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells. Lyso-Tracker indicated lysosomes, as the dye is highly selective for acidic environments, with an excitation wavelength of 577 nm and an emission wavelength of 590 nm. (F) Assessment of doxorubicin sensitivity changes in 17.94 NC, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells; n=3. The cell number ratio indicates the relative number of viable cells compared between initial cell number and different time points. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. UNC13B, unc-13 homolog B; NC, negative control; OE, over-expressed; KO, knock-out; LAMP1, lysosomal-associated membrane protein 1.

Journal: Oncology Letters

Article Title: UNC13B regulates the sensitivity of Wilms' tumor cells to doxorubicin by modulating lysosomes

doi: 10.3892/ol.2024.14579

Figure Lengend Snippet: UNC13B modulates cell drug sensitivity by affecting lysosome formation. (A) 17.94 cell line was transiently transfected with the UNC13B-pCDNA3.1 overexpression vector using Lipofectamine 3,000. Reverse transcription-quantitative PCR validation was performed 24 h post-transfection. NC was the transfection with an empty pcDNA3.1 vector; n=3. (B) UNC13B and LAMP1 expression levels in 17.94 NC cells, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells, using GAPDH as a reference, and (C) the associated semi-quantitative results. (D) Analysis of Mean Gray Value of Lyso-Tracker in 6 random fields of 17.94 NC, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells, and (E) representative images of 17.94 NC cells, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells. Lyso-Tracker indicated lysosomes, as the dye is highly selective for acidic environments, with an excitation wavelength of 577 nm and an emission wavelength of 590 nm. (F) Assessment of doxorubicin sensitivity changes in 17.94 NC, UNC13B 17.94 OE cells and UNC13B 17.94 KO cells; n=3. The cell number ratio indicates the relative number of viable cells compared between initial cell number and different time points. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. UNC13B, unc-13 homolog B; NC, negative control; OE, over-expressed; KO, knock-out; LAMP1, lysosomal-associated membrane protein 1.

Article Snippet: The negative controls are scramble sequences synthesized by igebio biotech Co., Ltd. UNC13B shRNA lentiviral vector using lentiCRISPR v2. (cat. no. 52961; Addgene, Inc.) was constructed.

Techniques: Transfection, Over Expression, Plasmid Preparation, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Negative Control, Knock-Out, Membrane

Effect of UNC13B gene ablation on the cell cycle in ARD cells. Five days after transduction with shRNA lentiviral vectors, the shUNC13B group showed a reduced fraction of ARD cells in G1 ( p < 0.0001) and an increased fraction in S phase ( p < 0.0001) compared with the Scramble control, with no significant change in G2/M (ns, not significant). Histograms show DNA-content modeling: red areas denote fitted G1 and G2/M peaks, the blue hatched area denotes S phase, and the black line is the measured distribution; ▲ indicates software-generated peak positions (reference only, not used for quantification). **** indicate p < 0.0001.

Journal: Biomedicines

Article Title: Inhibiting UNC13B Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma

doi: 10.3390/biomedicines13092086

Figure Lengend Snippet: Effect of UNC13B gene ablation on the cell cycle in ARD cells. Five days after transduction with shRNA lentiviral vectors, the shUNC13B group showed a reduced fraction of ARD cells in G1 ( p < 0.0001) and an increased fraction in S phase ( p < 0.0001) compared with the Scramble control, with no significant change in G2/M (ns, not significant). Histograms show DNA-content modeling: red areas denote fitted G1 and G2/M peaks, the blue hatched area denotes S phase, and the black line is the measured distribution; ▲ indicates software-generated peak positions (reference only, not used for quantification). **** indicate p < 0.0001.

Article Snippet: To investigate the functional role of UNC13B, two short hairpin RNA (shRNA) sequences targeting UNC13B (GenBank accession no.: NM_020313.2 ) were designed and cloned into the lentiviral vector lentiCRISPR v2 (Addgene plasmid #52961).

Techniques: Transduction, shRNA, Control, Software, Generated